Early or delayed time-restricted feeding prevents metabolic impact of obesity in mice

Time-restricted feeding (TRF) initiated early during the dark phase prevents metabolic consequences of a high-fat diet in rodent models. However, delaying initiation TRF, akin to breakfast skipping humans, is unclear. We assigned 8-week-old male C57BL/6J mice ( n = 192) chow or ad libitum (AL) for 4 weeks, before randomization continue AL 10 h at lights off (TRFe) 4-h after (TRFd) further 8 weeks. Oral glucose tolerance tests (1 g/kg), monitoring and body composition by echoMRI were performed, tissues collected six time points. TRF reduced weight fat mass vs AL, with greater reduction TRFe TRFd. improved protected from diet-induced hepatosteatosis no difference between increased amplitude Bmal1 , Cry1 Per2 Nampt Nocturnin mRNA levels liver. A delay Reverb? NAD, Sirt1 was observed Thus, limited benefit induced hepatic clock, but health. Allowing more flexibility when may increase translational potential this dietary approach humans.